{"id":1110,"date":"2022-08-23T15:56:43","date_gmt":"2022-08-23T18:56:43","guid":{"rendered":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/?p=1110"},"modified":"2022-08-24T10:40:33","modified_gmt":"2022-08-24T13:40:33","slug":"scientists-develop-3d-lung-alveoli-to-test-drugs","status":"publish","type":"post","link":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/7\/scientists-develop-3d-lung-alveoli-to-test-drugs\/","title":{"rendered":"Scientists develop 3D lung alveoli to test drugs"},"content":{"rendered":"
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A group of scientists from the Pel\u00e9 Pequeno Pr\u00edncipe Research Institute, led by researcher Dr. Katherine Athayde Teixeira de Carvalho, is developing a 3D pulmonary alveolus that could be used in the future to test new drugs that fight lung diseases. The project is part of the master\u2019s research by student N\u00e1dia Nascimento da Rosa, from the Pequeno Pr\u00edncipe College. With drug testing in the 3D alveolus, the use of animals is minimized, in addition to obtaining a model that is closer to the similarity of the human organism.<\/p>\n\n\n\n

The project emerged at the beginning of the coronavirus pandemic. \u201cThe first strains of SARS-CoV-2 affected the lungs very intensely. We then realized the need to be able to test new drugs in vitro<\/em>,and it was from there that we started to study the possibility of developing the alveolus, which is a functional unit of the lung,\u201d highlights the scientist.<\/p>\n\n\n\n

She explains that, although the lung has a varied cellular composition, with more than 40 cell types, the epithelial cells are the ones that concentrate the main functions of this organ. Based on this information, the team looked for a way to develop these cells. \u201cThis was possible through the use of umbilical cord stem cells, which differentiate into lung tissue cells. In the project, we are using 14 samples of umbilical cord stem cells,\u201d she reports.<\/p>\n\n\n\n

The developed differentiated cells are then transferred in an orderly fashion to a bioprinter. \u201cThis printer allows the cells to be arranged according to the organization of the lung tissue, particularly the alveolus,\u201d she points out.<\/p>\n\n\n

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After the tissue is bioprinted, the culture is transferred to the InCell Analyzer equipment, which allows the evaluation of cell viability. With the stratification of the tissue already established, a vascularization system is created in this tissue using epithelial cells. The histological organization of the tissue grown on the 3D printer is evaluated by immunocytochemistry to prove the characteristics of alveolar tissue, as well as scanning electron microscopy for the interaction of cells with the matrix.<\/p>\n\n\n\n

\u201cWith this methodology, we were able to prove that it is possible to differentiate stem cells into pneumocytes I and II cells and print the alveoli, making our 3D model functional \u2013 it, in fact, performs gas exchange, which makes our prototype suitable for testing drugs that act on the alveoli,\u201d she concludes.<\/p>\n","protected":false},"excerpt":{"rendered":"

Using a bioprinter, the researchers were able to reproduce the lung structure, making the prototype suitable for testing new drugs<\/p>\n","protected":false},"author":2,"featured_media":1123,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[8],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/wp-json\/wp\/v2\/posts\/1110"}],"collection":[{"href":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/wp-json\/wp\/v2\/comments?post=1110"}],"version-history":[{"count":8,"href":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/wp-json\/wp\/v2\/posts\/1110\/revisions"}],"predecessor-version":[{"id":1151,"href":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/wp-json\/wp\/v2\/posts\/1110\/revisions\/1151"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/wp-json\/wp\/v2\/media\/1123"}],"wp:attachment":[{"href":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/wp-json\/wp\/v2\/media?parent=1110"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/wp-json\/wp\/v2\/categories?post=1110"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pequenoprincipe.org.br\/newsano6\/en\/wp-json\/wp\/v2\/tags?post=1110"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}